Publications
Reviews are in green.
Gallegos LL, Newton AC.
Spatiotemporal dynamics of lipid signaling: Protein kinase C as a paradigm.
IUBMB Life. 2008 Aug 20. [Epub ahead of print]
Gould, CM, Newton AC.
The Life and Death of Protein Kinase C.
Curr Drug Targets. 2008 Aug;9(8):614-25. PDF
Facchinetti V, Ouyang W, Wei H, Soto N, Lazorchak A, Gould C, Lowry C, Newton AC, Mao Y, Miao RQ, Sessa WC, Qin J, Zhang P, Su B, Jacinto E.
The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C.
EMBO J. 2008 Jul 23;27(14):1932-43. PDF
Brognard J, Newton AC.
PHLPPing the switch on Akt and protein kinase C signaling.
Trends Endocrinol Metab. 2008 Aug;19(6):223-30. PDF
Dries DR, Newton AC.
Kinetic Analysis of the Interaction of the C1 Domain of Protein Kinase C with Lipid Membranes by Stopped-flow Spectroscopy.
J Biol Chem. 2008 Mar 21;283(12):7885-93. PDF
Gao T, Brognard J, Newton AC.
The phosphatase PHLPP controls the cellular levels of protein kinase C.
J Biol Chem. 2008 Mar 7;283(10):6300-6311. PDF
Newton AC, Mochly-Rosen D.
Induced Fit and Wit: Celebrating the Life of Daniel E. Koshland, Jr. (1920 - 2007).
IUBMB Life. 2007 Dec;59(12):741-3. PDF
Chen D, Gould C, Garza R, Gao T, Hampton RY, Newton AC.
Amplitude control of protein kinase C by RINCK, a novel E3 ubiquitin ligase.
J Biol Chem. 2007 Nov 16;282(46):33776-87. PDF
Brognard J, Sierecki E, Gao T, Newton AC.
PHLPP and a Second Isoform, PHLPP2, Differentially Attenuate the Amplitude of Akt Signaling by Regulating Distinct Akt Isoforms.
Mol Cell. 2007 Mar 23;25(6):917-31. PDF
Kunkel MT, Toker A, Tsien RY, Newton AC.
Calcium-dependent regulation of protein kinase D revealed by a genetically-encoded kinase activity reporter.
J Biol Chem. 2007 Mar 2;282(9):6733-42. PDF
Dries DR, Gallegos LL, Newton AC.
A single residue in the C1 domain sensitizes novel protein kinase C isoforms to cellular diacylglycerol production.
J. Biol. Chem. 2007 Jan 12; 282(2) 826-830. PDF
Gao T, Newton AC.
Invariant Leu preceding turn phosphorylation motif controls the interaction of protein kinase C with Hsp70
J Biol Chem. 2006 Oct 27;281(43):32461-8. PDF
Gallegos LL, Kunkel MT, Newton AC.
Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling.
J Biol Chem. 2006 Oct 13;281(41):30947-30956. PDF
Giorgione JR, Lin JH, McCammon JA, Newton AC.
Increased membrane affinity of the C1 domain of protein kinase Cdelta
compensates for the lack of involvement of its C2 domain in membrane
recruitment.
J Biol Chem. 2006 Jan 20;281(3):1660-9. PDF
Gao T, Furnari F, Newton AC.
PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth.
Mol Cell. 2005 Apr 1;18(1):13-24. PDF
Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC.
Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter.
J Biol Chem. 2005 Feb 18;280(7):5581-7. PDF
Newton AC
Protein Kinase C Family.
Encyclopedia of Biological Chemistry, 2004,3;523-526. PDF
King CC, Newton AC.
The adaptor protein Grb14 regulates the localization of 3-phosphoinositide-dependent kinase-1.
J Biol Chem. 2004 Sep 3;279(36):37518-27. PDF
Newton AC.
Diacylglycerol's affair with protein kinase C turns 25.
Trends Pharmacol Sci. 2004 Apr;25(4):175-7. PDF
Chen D, Purohit A, Halilovic E, Doxsey SJ, Newton AC.
Centrosomal anchoring of protein kinase C betaII by pericentrin
controls microtubule organization, spindle function, and cytokinesis.
J Biol Chem. 2004 Feb 6;279(6):4829-39. PDF
Violin JD, Newton AC.
Pathway illuminated: visualizing protein kinase C signaling.
IUBMB Life. 2003 Dec;55(12):653-60. PDF
Giorgione J, Hysell M, Harvey DF, Newton AC.
Contribution of the C1A and C1B domains to the membrane interaction of protein kinase C.
Biochemistry. 2003 Sep 30;42(38):11194-202. PDF
Kawakami Y, Kitaura J, Yao L, McHenry RW, Kawakami Y, Newton AC, Kang S, Kato RM, Leitges M, Rawlings DJ, Kawakami T.
A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9470-5.
Nalefski EA, Newton AC.
Use of stopped-flow fluorescence spectroscopy to measure rapid membrane binding by protein kinase C.
Methods Mol Biol. 2003;233:115-28. PDF
Giorgione J, Newton AC.
Measuring the binding of protein kinase C to sucrose-loaded vesicles.
Methods Mol Biol. 2003;233:105-13. PDF
Newton AC.
Measuring the interaction of protein kinase C with membranes: an introduction.
Methods Mol Biol. 2003;233:89-92. PDF
Newton AC.
The ins and outs of protein kinase C.
Methods Mol Biol. 2003;233:3-7. PDF
Bertolini TM, Giorgione J, Harvey DF, Newton AC.
Protein kinase C translocation by modified phorbol esters with functionalized lipophilic regions.
J Org Chem. 2003 Jun 27;68(13):5028-36. PDF
Adams RA, Liu X, Williams DS, Newton AC.
Differential spatial and temporal phosphorylation of the visual
receptor, rhodopsin, at two primary phosphorylation sites in mice
exposed to light.
Biochem J. 2003 Sep 1;374(Pt 2):537-43. PDF
Violin JD, Zhang J, Tsien RY, Newton AC.
A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C.
J Cell Biol. 2003 Jun 9;161(5):899-909. PDF
Newton AC.
Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm.
Biochem J. 2003 Mar 1;370(Pt 2):361-71. PDF
Newton AC
Protein Kinase C: Relaying Signals from Lipid Hydrolysis to Protein Phosphorylation.
In Handbook of Cell Signaling (Elsevier Science) 2003;2:187-192.
Gao T, Newton AC.
The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C.
J Biol Chem. 2002 Aug 30;277(35):31585-92. PDF
Zacharias DA, Violin JD, Newton AC, Tsien RY.
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
Science. 2002 May 3;296(5569):913-6. PDF
Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A.
Regulation of novel protein kinase C epsilon by phosphorylation.
Biochem J. 2002 May 1;363(Pt 3):537-45. PDF
Newton AC.
Analyzing protein kinase C activation.
Methods Enzymol. 2002;345:499-506.
Newton AC.
Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions.
Chem Rev. 2001 Aug;101(8):2353-64. PDF
Nalefski EA, Newton AC.
Membrane binding kinetics of protein kinase C betaII mediated by the C2 domain.
Biochemistry. 2001 Nov 6;40(44):13216-29. PDF
Perkins GA, Wang L, Huang LJ, Humphries K, Yao VJ, Martone M, Deerinck TJ, Barraclough DM, Violin JD, Smith D, Newton A, Scott JD, Taylor SS, Ellisman MH.
PKA, PKC, and AKAP localization in and around the neuromuscular junction.
BMC Neurosci. 2001 Oct 23;2:17. PDF
Newton AC, Toker A.
Cellular Regulation of Protein Kinase C.
In Protein Adaptations and Signal Transduction (Elsevier Science) 2001;163-173.
Sonnenburg ED, Gao T, Newton AC.
The
phosphoinositide-dependent kinase, PDK-1, phosphorylates conventional
protein kinase C isozymes by a mechanism that is independent of
phosphoinositide 3-kinase.
J Biol Chem. 2001 Nov 30;276(48):45289-97. PDF
Gao T, Toker A, Newton AC.
The carboxyl terminus
of protein kinase c provides a switch to regulate its interaction with
the phosphoinositide-dependent kinase, PDK-1.
J Biol Chem. 2001 Jun 1;276(22):19588-96. PDF
Toker A, Newton AC.
Cellular signaling: pivoting around PDK-1.
Cell. 2000 Oct 13;103(2):185-8. PDF
King CC, Gardiner EM, Zenke FT, Bohl BP, Newton AC, Hemmings BA, Bokoch GM.
p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).
J Biol Chem. 2000 Dec 29;275(52):41201-9. PDF
Johnson JE, Giorgione J, Newton AC.
The C1 and C2 domains of protein kinase C are independent membrane
targeting modules, with specificity for phosphatidylserine conferred by
the C1 domain.
Biochemistry. 2000 Sep 19;39(37):11360-9. PDF
Dempsey EC, Newton AC, Mochly-Rosen D, Fields AP, Reyland ME, Insel PA, Messing RO.
Protein kinase C isozymes and the regulation of diverse cell responses.
Am J Physiol Lung Cell Mol Physiol. 2000 Sep;279(3):L429-38. PDF
King CC, Zenke FT, Dawson PE, Dutil EM, Newton AC, Hemmings BA, Bokoch GM.
Sphingosine is a novel activator of 3-phosphoinositide-dependent kinase 1.
J Biol Chem. 2000 Jun 16;275(24):18108-13. PDF
Dutil EM, Newton AC.
Dual role of pseudosubstrate in the coordinated regulation of protein kinase C by phosphorylation and diacylglycerol.
J Biol Chem. 2000 Apr 7;275(14):10697-701. PDF
Granados-Soto V, Kalcheva I, Hua X, Newton A, Yaksh TL.
Spinal PKC activity and expression: role in tolerance produced by continuous spinal morphine infusion.
Pain. 2000 Apr;85(3):395-404. PDF
Toker A, Newton AC.
Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site.
J Biol Chem. 2000 Mar 24;275(12):8271-4. PDF
Newton AC
Protein Kinase C.
In Principles of Molecular Regulation (Humana Press) 2000;207-220.
Faux MC, Rollins EN, Edwards AS, Langeberg LK, Newton AC, Scott JD.
Mechanism of A-kinase-anchoring protein 79 (AKAP79) and protein kinase C interaction.
Biochem J. 1999 Oct 15;343 Pt 2:443-52.
Behn-Krappa A, Newton AC.
The hydrophobic phosphorylation motif of conventional protein kinase C is regulated by autophosphorylation.
Curr Biol. 1999 Jul 15;9(14):728-37.
Edwards AS, Faux MC, Scott JD, Newton AC.
Carboxyl-terminal phosphorylation regulates the function and subcellular localization of protein kinase C betaII.
J Biol Chem. 1999 Mar 5;274(10):6461-8.
Dutil EM, Toker A, Newton AC.
Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1).
Curr Biol. 1998 Dec 17-31;8(25):1366-75.
Mosior M, Newton AC.
Mechanism of the apparent cooperativity in the interaction of protein kinase C with phosphatidylserine.
Biochemistry. 1998 Dec 8;37(49):17271-9.
Chou MM, Hou W, Johnson J, Graham LK, Lee MH, Chen CS, Newton AC, Schaffhausen BS, Toker A.
Regulation of protein kinase C zeta by PI 3-kinase and PDK-1.
Curr Biol. 1998 Sep 24;8(19):1069-77.
Newton AC, Johnson JE.
Protein kinase C: a paradigm for regulation of protein function by two membrane-targeting modules.
Biochim Biophys Acta. 1998 Aug 21;1376(2):155-72.
Johnson JE, Zimmerman ML, Daleke DL, Newton AC.
Lipid structure and not membrane structure is the major determinant in
the regulation of protein kinase C by phosphatidylserine.
Biochemistry. 1998 Sep 1;37(35):12020-5.
Udovichenko IP, Newton AC, Williams DS.
Regulation of the phosphorylation state of rhodopsin by dopamine.
J Biol Chem. 1998 Mar 27;273(13):7181-4.
Edwards AS, Newton AC.
Regulation of protein kinase C betaII by its C2 domain.
Biochemistry. 1997 Dec 16;36(50):15615-23.
Johnson JE, Edwards AS, Newton AC.
A putative phosphatidylserine binding motif is not involved in the lipid regulation of protein kinase C.
J Biol Chem. 1997 Dec 5;272(49):30787-92.
Williams DS, Liu X, Schlamp CL, Ondek B, Jaken S, Newton AC.
Characterization of protein kinase C in photoreceptor outer segments.
J Neurochem. 1997 Oct;69(4):1693-702.
Keranen LM, Newton AC.
Ca2+ differentially regulates conventional protein kinase Cs' membrane interaction and activation.
J Biol Chem. 1997 Oct 10;272(41):25959-67.
Edwards AS, Newton AC.
Phosphorylation at
conserved carboxyl-terminal hydrophobic motif regulates the catalytic
and regulatory domains of protein kinase C.
J Biol Chem. 1997 Jul 18;272(29):18382-90.
Greene NM, Williams DS, Newton AC.
Identification of protein kinase C phosphorylation sites on bovine rhodopsin.
J Biol Chem. 1997 Apr 18;272(16):10341-4.
Newton AC.
Regulation of protein kinase C.
Curr Opin Cell Biol. 1997 Apr;9(2):161-7.
Udovichenko IP, Newton AC, Williams DS.
Contribution of protein kinase C to the phosphorylation of rhodopsin in intact retinas.
J Biol Chem. 1997 Mar 21;272(12):7952-9.
Hurley JH, Newton AC, Parker PJ, Blumberg PM, Nishizuka Y.
Taxonomy and function of C1 protein kinase C homology domains.
Protein Sci. 1997 Feb;6(2):477-80.
Arnold RS, Newton AC.
Inhibition of the insulin receptor tyrosine kinase by phosphatidic acid.
J Cell Biochem. 1996 Sep 15;62(4):516-28.
Newton AC.
Protein kinase C: ports of anchor in the cell.
Curr Biol. 1996 Jul 1;6(7):806-9. PDF
Arnold RS, Newton AC.
Diacylglycerol directly stimulates the insulin receptor tyrosine kinase.
FEBS Lett. 1996 Feb 12;380(1-2):58-62.
Mosior M, Newton AC.
Calcium-independent binding to interfacial phorbol esters causes
protein kinase C to associate with membranes in the absence of acidic
lipids.
Biochemistry. 1996 Feb 6;35(5):1612-23.
Keranen LM, Dutil EM, Newton AC.
Protein kinase C is regulated in vivo by three functionally distinct phosphorylations.
Curr Biol. 1995 Dec 1;5(12):1394-1403. PDF
Newton AC.
Protein kinase C: structure, function, and regulation.
J Biol Chem. 1995 Dec 1;270(48):28495-8.
Mosior M, Newton AC.
Mechanism of interaction of protein kinase C with phorbol esters. Reversibility and nature of membrane association.
J Biol Chem. 1995 Oct 27;270(43):25526-33.
Newton AC.
Protein kinase C. Seeing two domains.
Curr Biol. 1995 Sep 1;5(9):973-6.
Greene NM, Williams DS, Newton AC.
Kinetics and localization of the phosphorylation of rhodopsin by protein kinase C.
J Biol Chem. 1995 Mar 24;270(12):6710-7.
Conrad R, Keranen LM, Ellington AD, Newton AC.
Related Articles, Links
Isozyme-specific inhibition of protein kinase C by RNA aptamers.
J Biol Chem. 1994 Dec 23;269(51):32051-4.
Dutil EM, Keranen LM, DePaoli-Roach AA, Newton AC.
In vivo regulation of protein kinase C by trans-phosphorylation followed by autophosphorylation.
J Biol Chem. 1994 Nov 25;269(47):29359-62.
Orr JW, Newton AC.
Requirement for negative charge on "activation loop" of protein kinase C.
J Biol Chem. 1994 Nov 4;269(44):27715-8.
Williams DS, Park S, Schlamp CL, Newton AC.
Protein kinase C association with the retinal cytoskeleton and phosphorylation of vimentin.
Exp Eye Res. 1994 Jun;58(6):747-59.
Newton AC, Keranen LM.
Phosphatidyl-L-serine is necessary for protein kinase C's high-affinity
interaction with diacylglycerol-containing membranes.
Biochemistry. 1994 May 31;33(21):6651-8.
Orr JW, Newton AC.
Intrapeptide regulation of protein kinase C.
J Biol Chem. 1994 Mar 18;269(11):8383-7.
Daleke DL, Huestis WH, Newton AC.
Protein kinase C as a measure of transbilayer phosphatidylserine asymmetry.
Anal Biochem. 1994 Feb 15;217(1):33-40.
Nickells RW, Schlamp CL, Newton AC, Williams DS.
Gene expression of the neuropeptide-processing enzyme carboxypeptidase E in rat photoreceptor cells.
J Neurochem. 1993 Nov;61(5):1891-900.
Newton AC, Williams DS.
Rhodopsin is the major in situ substrate of protein kinase C in rod outer segments of photoreceptors.
J Biol Chem. 1993 Aug 25;268(24):18181-6.
Newton AC, Williams DS.
Does protein kinase C play a role in rhodopsin desensitization?
Trends Biochem Sci. 1993 Aug;18(8):275-7.
Newton AC.
Interaction of proteins with lipid headgroups: lessons from protein kinase C.
Annu Rev Biophys Biomol Struct. 1993;22:1-25
Orr JW, Keranen LM, Newton AC.
Reversible exposure of the pseudosubstrate domain of protein kinase C by phosphatidylserine and diacylglycerol.
J Biol Chem. 1992 Aug 5;267(22):15263-6.
Orr JW, Newton AC.
Interaction of protein kinase C with phosphatidylserine. 2. Specificity and regulation.
Biochemistry. 1992 May 19;31(19):4667-73.
Orr JW, Newton AC.
Interaction of protein kinase C with phosphatidylserine. 1. Cooperativity in lipid binding.
Biochemistry. 1992 May 19;31(19):4661-7.
Newton AC, Williams DS.
Involvement of protein kinase C in the phosphorylation of rhodopsin.
J Biol Chem. 1991 Sep 25;266(27):17725-8.
Arnold RS, Newton AC.
Inhibition of the insulin receptor tyrosine kinase by sphingosine.
Biochemistry. 1991 Aug 6;30(31):7747-54.
Newton AC, Koshland DE Jr.
Phosphatidylserine affects specificity of protein kinase C substrate phosphorylation and autophosphorylation.
Biochemistry. 1990 Jul 17;29(28):6656-61.
Newton AC, Koshland DE Jr.
High cooperativity, specificity, and multiplicity in the protein kinase C-lipid interaction.
J Biol Chem. 1989 Sep 5;264(25):14909-15.
Newton AC, Koshland DE Jr.
Protein kinase C autophosphorylates by an intrapeptide reaction.
J Biol Chem. 1987 Jul 25;262(21):10185-8.
